Screening and sequencing monoclonal antibody at single-cell level

Weikai Zhang; Zewen Wei; Qin Li

doi:10.1117/12.2601468

9 October 2021 Screening and sequencing monoclonal antibody at single-cell level

Weikai Zhang, Zewen Wei, Qin Li

Proceedings Volume 11900, Optics in Health Care and Biomedical Optics XI; 1190018 (2021) https://doi.org/10.1117/12.2601468
Event: SPIE/COS Photonics Asia, 2021, Nantong, JS, China

Abstract

Acquisition of the genes encoding variable regions of paired heavy and light chains (VH:VL) is crucial, but it is a labor and cost-intensive process in traditional methods. This study presents a novel method in which all processing steps for acquiring natively paired VH:VL genes from single cells are finished in a single microfluidic chip. The microfluidic chip performs single-cell trap/in situ fluorescent examination of antibody specificity/cell lysis/gene amplification all at single-cell level. By a proof-of-concept validation of efficiently acquiring paired VH:VL genes of anti-RBD (which is a key protein of SARS-CoV-2 virus) mAbs from single hybridomas, the microfluidic chip has been proved capable of remarkably improving cell loss/human labor/time cost, and more importantly, determinacy of native VH:VL genes pairing which is one of the most decisive factors of effectiveness for antibody discovery.

Conference Presentation

Citation Download Citation

Weikai Zhang, Zewen Wei, and Qin Li "Screening and sequencing monoclonal antibody at single-cell level", Proc. SPIE 11900, Optics in Health Care and Biomedical Optics XI, 1190018 (9 October 2021); https://doi.org/10.1117/12.2601468

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