Investigation of plant cells intracellular dynamics by digital holography

Zhe Wang; Vittorio Bianco; Daniele Pirone; Pasquale Memmolo; Massimiliano Maria Villone; Pier Luca Maffettone; Pietro Ferraro

doi:10.1117/12.2592678

20 June 2021 Investigation of plant cells intracellular dynamics by digital holography

Zhe Wang, Vittorio Bianco, Daniele Pirone, Pasquale Memmolo, Massimiliano Maria Villone, Pier Luca Maffettone, Pietro Ferraro

Author Affiliations +

Proceedings Volume 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V; 117860M (2021) https://doi.org/10.1117/12.2592678
Event: SPIE Optical Metrology, 2021, Online Only

Abstract

Visualizing the intracellular dynamics of plant cells has been an open challenge for modern botany, agronomy and pharmacy. In this paper, we proposed an approach to improve the phase contrast during plant cell holographic imaging by cells’ dehydration, and used this method to realize the observation of cytoplasmic circulation inside the living onion epithelial cell. The dehydration process can be seen as a sort of label-free contrast agent for better imaging biological processes. We have investigated live onion epidermal cells, observing their inner dynamics during long time recordings using a digital holographic microscopy system. For the experiments, an off-axis digital holography setup in transmission configuration with double spherical wave interference was used to record the digital holograms of onion cells. Then, we performed long-term time lapse holographic recordings of onion epidermal cells, and the results show that the intracellular tissue structure and the dynamic behavior of the cytoskeleton features and nuclei can be better exhibited via high-contrast phase imaging under cell dehydration conditions. In this case, the movements of intracellular filaments and the nucleus are observed via dynamical high-contrast phase imaging during the dehydration process. The experimental results clearly show the positive effect of dehydration process on intracellular imaging quality, and create the possibility to track the movement of plant organelles. In sum, thanks to the dehydration process of plant cells, holographic phase contrast enhancement imaging is realized.

Conference Presentation

Citation Download Citation

Zhe Wang, Vittorio Bianco, Daniele Pirone, Pasquale Memmolo, Massimiliano Maria Villone, Pier Luca Maffettone, and Pietro Ferraro "Investigation of plant cells intracellular dynamics by digital holography", Proc. SPIE 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V, 117860M (20 June 2021); https://doi.org/10.1117/12.2592678

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available