Research on improving reconstruction quality from holograms of marine algae in digital holographic microscopy

Zhiyuan Tang; Ming Tang; Xin Qiao; Kaiqi Lang; Xiaoping Wang

doi:10.1117/12.2586391

12 March 2021 Research on improving reconstruction quality from holograms of marine algae in digital holographic microscopy

Zhiyuan Tang, Ming Tang, Xin Qiao, Kaiqi Lang, Xiaoping Wang

Author Affiliations +

Proceedings Volume 11763, Seventh Symposium on Novel Photoelectronic Detection Technology and Applications; 117632G (2021) https://doi.org/10.1117/12.2586391
Event: Seventh Symposium on Novel Photoelectronic Detection Technology and Application 2020, 2020, Kunming, China

Abstract

In this paper, we improve reconstruction quality from in-line holograms of marine algae by two phase-retrieval-based reconstruction methods. Digital holographic microscopy, with its high-resolution and volume recording capability, has been applied in the area of monitoring marine algae. We designed a digital in-line holographic microscopy laboratory system to observe and analyze marine algae. Due to the “twin-image” problem caused by the loss of phase information in hologram recording, the reconstructed quality from holograms of marine algae reduced significantly. To enhance reconstruction quality, the alternating projections methods and inverse methods are introduced and implemented on the marine algae holograms. Compared with the traditional backpropagation method, both of the above methods have good performance in improving reconstruction quality from in-line marine algae holograms.

Citation Download Citation

Zhiyuan Tang, Ming Tang, Xin Qiao, Kaiqi Lang, and Xiaoping Wang "Research on improving reconstruction quality from holograms of marine algae in digital holographic microscopy", Proc. SPIE 11763, Seventh Symposium on Novel Photoelectronic Detection Technology and Applications, 117632G (12 March 2021); https://doi.org/10.1117/12.2586391

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