Development of a fast, large area multiphoton exoscope (FLAME) for clinical skin imaging

Mihaela Balu; Alexander Fast; Amanda Durkin; Griffin Lentsch

doi:10.1117/12.2582795

5 March 2021 Development of a fast, large area multiphoton exoscope (FLAME) for clinical skin imaging

Mihaela Balu, Alexander Fast, Amanda Durkin, Griffin Lentsch

Proceedings Volume 11654, High-Speed Biomedical Imaging and Spectroscopy VI; 116540C (2021) https://doi.org/10.1117/12.2582795
Event: SPIE BiOS, 2021, Online Only

Abstract

Multiphoton microscopy enables sub-micron resolution, label-free structural and functional imaging of living tissues with contrast from multiple modalities, including second harmonic generation and two-photon excited fluorescence. We developed a fast, large area multiphoton exoscope (FLAME) portable system with enhanced label-free molecular contrast for macroscopic imaging of human skin with microscopic resolution. It combines optical and mechanical scanning mechanisms with deep learning image restoration to produce 3D sub-cellular resolution images that encompass sub-mm2 to cm2 scale areas of tissue within minutes. We demonstrate the performance and utility of the instrument by fast ex vivo and in vivo imaging of human skin.

Conference Presentation

Citation Download Citation

Mihaela Balu, Alexander Fast, Amanda Durkin, and Griffin Lentsch "Development of a fast, large area multiphoton exoscope (FLAME) for clinical skin imaging", Proc. SPIE 11654, High-Speed Biomedical Imaging and Spectroscopy VI, 116540C (5 March 2021); https://doi.org/10.1117/12.2582795

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