Automated single-shot sensorless adaptive optics on a multimodal imaging platform using computational adaptive optics

Rishyashring R. Iyer; Janet E. Sorrells; Lingxiao Yang; Mantas Žurauskas; Stephen A. Boppart

doi:10.1117/12.2576991

5 March 2021 Automated single-shot sensorless adaptive optics on a multimodal imaging platform using computational adaptive optics

Rishyashring R. Iyer, Janet E. Sorrells, Lingxiao Yang, Mantas Žurauskas, Stephen A. Boppart

Author Affiliations +

Proceedings Volume 11652, Adaptive Optics and Wavefront Control for Biological Systems VII; 116520C (2021) https://doi.org/10.1117/12.2576991
Event: SPIE BiOS, 2021, Online Only

Abstract

Multimodal imaging systems are designed to extract complementary multifaceted information from biological samples; sensorless adaptive optics (AO) seeks to extend their capabilities by estimating the wavefront from image-derived information. Typical sensorless AO techniques need the acquisition of several volumes for optimization. We present a novel single-shot closed-loop sensorless AO technique demonstrated on a label-free multimodal imaging system consisting of optical coherence, two-photon fast fluorescence lifetime imaging, and second harmonic generation microscopy. The wavefront is sensed by performing computational AO on an initial OCM volume that is translated to the deformable mirror to improve the image quality of all modalities in real-time.

Conference Presentation

Citation Download Citation

Rishyashring R. Iyer, Janet E. Sorrells, Lingxiao Yang, Mantas Žurauskas, and Stephen A. Boppart "Automated single-shot sensorless adaptive optics on a multimodal imaging platform using computational adaptive optics", Proc. SPIE 11652, Adaptive Optics and Wavefront Control for Biological Systems VII, 116520C (5 March 2021); https://doi.org/10.1117/12.2576991

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available