Hybrid open-top light-sheet microscopy for 3D imaging of cleared tissues with mesoscopic and sub-micron resolution

Adam K. Glaser; Jonathan Liu

doi:10.1117/12.2577711

5 March 2021 Hybrid open-top light-sheet microscopy for 3D imaging of cleared tissues with mesoscopic and sub-micron resolution

Adam K. Glaser, Jonathan Liu

Author Affiliations +

Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 116490O (2021) https://doi.org/10.1117/12.2577711
Event: SPIE BiOS, 2021, Online Only

Abstract

Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need for a user-friendly system that can address diverse imaging applications with varied requirements in terms of resolution (mesoscopic to sub-micron), sample geometry (size, shape, and number), and compatibility with tissue-clearing protocols of different refractive indices. We present a hybrid system that combines a novel non-orthogonal dual-objective and conventional open-top light-sheet architecture for highly versatile multi-scale volumetric imaging.

Conference Presentation

Citation Download Citation

Adam K. Glaser and Jonathan Liu "Hybrid open-top light-sheet microscopy for 3D imaging of cleared tissues with mesoscopic and sub-micron resolution", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 116490O (5 March 2021); https://doi.org/10.1117/12.2577711

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