Characterizing three-dimensional micromechanical heterogeneities of the extracellular matrix with photonic-force optical coherence elastography

Yuechuan Lin; Nichaluk Leartprapun; Justin C. Luo; Steven G Adie

doi:10.1117/12.2583945

5 March 2021 Characterizing three-dimensional micromechanical heterogeneities of the extracellular matrix with photonic-force optical coherence elastography

Yuechuan Lin, Nichaluk Leartprapun, Justin C. Luo, Steven G Adie

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Proceedings Volume 11645, Optical Elastography and Tissue Biomechanics VIII; 1164505 (2021) https://doi.org/10.1117/12.2583945
Event: SPIE BiOS, 2021, Online Only

Abstract

We demonstrate applications of photonic-force optical coherence elastography (PF-OCE) on imaging three-dimensional (3D) micro-scale mechanical heterogeneity in collagen phantoms and cell-modified extracellular matrix. In collagen, we observed strong correlations between the measured mechanical heterogeneity and local OCT intensity in the vicinity of beads, which could be related to the presence of fibrous structures. Moreover, we quantitatively imaged micro-scale localized stiffening and increased heterogeneities in fibers surrounding an isolated NIH-3T3 fibroblast enclosed in a 2.5 mg/mL fibrin gel with a volumetric field-of-view (FOV) of ~80μm×350μm×50μm. Our results show that PF-OCE is able to characterize 3D micromechanical properties of biomaterials, and has the potential to observe dynamics of cell-ECM interactions.

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Citation Download Citation

Yuechuan Lin, Nichaluk Leartprapun, Justin C. Luo, and Steven G Adie "Characterizing three-dimensional micromechanical heterogeneities of the extracellular matrix with photonic-force optical coherence elastography", Proc. SPIE 11645, Optical Elastography and Tissue Biomechanics VIII, 1164505 (5 March 2021); https://doi.org/10.1117/12.2583945

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KEYWORDS

Coherence (optics)

Elastography

Optical coherence tomography

3D image processing

Cancer

Collagen

Image resolution

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