Comparison between dynamic microscopic OCT and autofluorescence multiphoton microscopy for label-free analysis of murine trachea

Tabea Kohlfaerber; Michael Münter; Mario Pieper; Peter König; Ramtin Rahmanzadeh; Gereon Hüttmann; Hinnerk Schulz-Hildebrandt

doi:10.1117/12.2583811

5 March 2021 Comparison between dynamic microscopic OCT and autofluorescence multiphoton microscopy for label-free analysis of murine trachea

Tabea Kohlfaerber, Michael Münter, Mario Pieper, Peter König, Ramtin Rahmanzadeh, Gereon Hüttmann, Hinnerk Schulz-Hildebrandt

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Proceedings Volume 11630, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV; 116300S (2021) https://doi.org/10.1117/12.2583811
Event: SPIE BiOS, 2021, Online Only

Abstract

Optical coherence tomography (OCT) with axial and lateral resolution of 1 µm, termed microscopic OCT (mOCT), is suited for the investigation of dynamic processes on cellular level. An improvement in contrast to visualize cellular structures can be achieved by evaluating inherent signal fluctuation. This so-called dynamic contrast was recently demonstrated for the widely used scanning frequency domain OCT (FD-OCT). Here we show comparative measurements of dynamic microscopic OCT (dmOCT) and multiphoton autofluorescence imaging of ex-vivo trachea. Compared to multiphoton microscopy, the dmOCT provides greater penetration depth, can visualize structures that are not detectable by autofluorescence imaging and has no risk of photodamage.

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Tabea Kohlfaerber, Michael Münter, Mario Pieper, Peter König, Ramtin Rahmanzadeh, Gereon Hüttmann, and Hinnerk Schulz-Hildebrandt "Comparison between dynamic microscopic OCT and autofluorescence multiphoton microscopy for label-free analysis of murine trachea", Proc. SPIE 11630, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV, 116300S (5 March 2021); https://doi.org/10.1117/12.2583811

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KEYWORDS

Optical coherence tomography

Multiphoton microscopy

Image resolution

Visualization

Microscopy

Multiphoton fluorescence microscopy

Objectives

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