Wenjun Shao,1 Kivilcim Kilic,1 Wenqing Yin,1 Gregory Wirak,1 Xiaodan Qin,1 Hui Feng,1 David Boashttps://orcid.org/0000-0002-6709-7711,1 Christopher V. Gabel,1 Ji Yi1
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Most reports on oblique plane microscopy (OPM) have a limited microscopic field of view (FOV), typically within 1x1 mm2. We implemented an optical design of mesoscopic scanning oblique plane microscopy (OPM) to allow using low numerical aperture (NA) objective lens. The angle of the intermediate image before the remote focusing system was increased by a demagnification under Scheimpflug condition such that the light collecting efficiency in the remote focusing system was significantly improved. We demonstrate a mesoscopic FOV up to ~6× 5×0.6 mm3 volumetric imaging, the largest reported FOV by OPM so far.
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Wenjun Shao, Kivilcim Kilic, Wenqing Yin, Gregory Wirak, Xiaodan Qin, Hui Feng, David Boas, Christopher V. Gabel, Ji Yi, "Wide field-of-view volumetric imaging by a mesoscopic scanning oblique plane microscopy," Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 116291V (5 March 2021); https://doi.org/10.1117/12.2577414