Quantitative detection of protoporphyrin IX (PpIX) fluorescence in tissues

Jian Zou; Na Meng; Weijun Li; Shusen Xie; Chunfa Wu; Zheng Huang

doi:10.1117/12.2575223

10 October 2020 Quantitative detection of protoporphyrin IX (PpIX) fluorescence in tissues

Jian Zou, Na Meng, Weijun Li, Shusen Xie, Chunfa Wu, Zheng Huang

Proceedings Volume 11553, Optics in Health Care and Biomedical Optics X; 115530V (2020) https://doi.org/10.1117/12.2575223
Event: SPIE/COS Photonics Asia, 2020, Online Only

Abstract

In situ quantification of photosensitizer is critical in photodynamic therapy (PDT) and photodiagnosis (PD). Fluorescence detection is a feasible approach for the quantification of fluorescent photosensitizer. However, due to the interference of tissue absorption and scattering on the fluorescence spectrum of photosensitizer, it is still challenging to perform in situ fluorescence quantification. In this preliminary study, a Monte Carlo (MC)-based method was used to simulate the fluorescence spectrum and diffuse reflection spectrum of different biological tissues. A calibration algorithm was developed for the correction of the influence of tissue absorption and scattering on protoporphyrin IX (PpIX) fluorescence. Under the excitation of blue light of 405 nm the dispersion coefficient of the original PpIX fluorescence spectrum of the soft tissue phantoms was 28%, which was reduced to 3% after the correction using the calibration algorithm. Under the excitation of red light of 635 nm, the dispersion coefficient of the original PpIX fluorescence spectrum of the soft tissue phantoms was 25%, which was reduced to 1.5% after the correction using the calibration algorithm. The results show that the MC-based method can effectively improve the accuracy of PpIX fluorescence measurement.

Conference Presentation

Citation Download Citation

Jian Zou, Na Meng, Weijun Li, Shusen Xie, Chunfa Wu, and Zheng Huang "Quantitative detection of protoporphyrin IX (PpIX) fluorescence in tissues", Proc. SPIE 11553, Optics in Health Care and Biomedical Optics X, 115530V (10 October 2020); https://doi.org/10.1117/12.2575223

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