Developments of infrared-pump visible-probe phase imaging on chemical selectivity and deep-learning enhancement

Weiru Fan; Tianrun Chen; Vlad V. Yakovlev; Dawei Wang; Delong Zhang

doi:10.1117/12.2575404

10 October 2020 Developments of infrared-pump visible-probe phase imaging on chemical selectivity and deep-learning enhancement

Weiru Fan, Tianrun Chen, Vlad V. Yakovlev, Dawei Wang, Delong Zhang

Author Affiliations +

Proceedings Volume 11549, Advanced Optical Imaging Technologies III; 115490T (2020) https://doi.org/10.1117/12.2575404
Event: SPIE/COS Photonics Asia, 2020, Online Only

Abstract

Optical phase microscopy is widely adopted for quantitative imaging of optical density in transparent cells and tissues, yet lacks the chemical selectivity. To address this challenge, a bond-selective transient phase imaging (BTSP) technique was developed, in which a transient change in phase induced by infrared excitation of molecular vibrations was detected by a diffraction phase microscope. BTSP achieved chemically selective phase imaging of live cells. We further demonstrated an IR-pump visible-probe phase microscopy based on second harmonic generation after the sample, enabled by deep learning. The phase-sensitive information is encoded into the second harmonic signal, which is decoded using a deep learning algorithm. It presents a label-free technique featured by high phase sensitivity and high robustness against noises, which has promising applications in biological and medical imaging and remote sensing.

Conference Presentation

Citation Download Citation

Weiru Fan, Tianrun Chen, Vlad V. Yakovlev, Dawei Wang, and Delong Zhang "Developments of infrared-pump visible-probe phase imaging on chemical selectivity and deep-learning enhancement", Proc. SPIE 11549, Advanced Optical Imaging Technologies III, 115490T (10 October 2020); https://doi.org/10.1117/12.2575404

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Phase imaging

Infrared imaging

Infrared radiation

Algorithm development

Harmonic generation

Microscopy

Absorbance

Show All Keywords

Keywords/Phrases

Search In:

Publication Years