Determination of local cell membrane slope fluctuations using the pitch rotational mode of optical tweezers

Rahul Vaippully; Vaibavi Ramanujam; Manoj Gopalakrishnan; Saumendra Bajpai; Basudev Roy

doi:10.1117/12.2563614

20 August 2020 Determination of local cell membrane slope fluctuations using the pitch rotational mode of optical tweezers

Rahul Vaippully, Vaibavi Ramanujam, Manoj Gopalakrishnan, Saumendra Bajpai, Basudev Roy

Author Affiliations +

Proceedings Volume 11463, Optical Trapping and Optical Micromanipulation XVII; 1146315 (2020) https://doi.org/10.1117/12.2563614
Event: SPIE Nanoscience + Engineering, 2020, Online Only

Abstract

We study the normal fluctuations of an MCF-7 cell membrane and calibrate the optical trap to detect pitch motion to get information about the rocking motion of a birefringent particle. We could show both theoretically and experimentally that the Z power spectrum has a power-law behavior of (frequency)^−5/3, and We find that the power spectrum of slope fluctuations is proportional to (frequency)⁻¹. We could extract parameters like bending rigidity directly from the power spectrum fitting parameters in 5 Hz to 1 kHz range. Our method was powerful enough to identify pitch rotation for a spherical birefringent particle to a high resolution using optical tweezers.

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Citation Download Citation

Rahul Vaippully, Vaibavi Ramanujam, Manoj Gopalakrishnan, Saumendra Bajpai, and Basudev Roy "Determination of local cell membrane slope fluctuations using the pitch rotational mode of optical tweezers", Proc. SPIE 11463, Optical Trapping and Optical Micromanipulation XVII, 1146315 (20 August 2020); https://doi.org/10.1117/12.2563614

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