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9 April 2020 Hydrothermal treatment of biotin molecule
Alina A. Kokorina, Irina Yu. Goryacheva
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Proceedings Volume 11457, Saratov Fall Meeting 2019: Optical and Nano-Technologies for Biology and Medicine; 114571I (2020) https://doi.org/10.1117/12.2561253
Event: Saratov Fall Meeting 2019: VII International Symposium on Optics and Biophotonics, 2019, Saratov, Russian Federation
Abstract
We demonstrate the synthesis of a luminescent derivative of biotin molecules with preservation its ability to interact with streptavidin. The luminescent biotin derivative was obtained via hydrothermal treatment of biotin water solution at different temperatures 140-240°C for 2 hours. Modified biotin showed a strong blue emission in the area of 390 nm with an optimal excitation wavelength at 320 nm and the relative quantum yield was 15±2 % for the sample obtained at the highest temperature. It was shown that hydrothermal treatment leads to biotin modifying with a saving of its biological activity.
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Alina A. Kokorina and Irina Yu. Goryacheva "Hydrothermal treatment of biotin molecule", Proc. SPIE 11457, Saratov Fall Meeting 2019: Optical and Nano-Technologies for Biology and Medicine, 114571I (9 April 2020); https://doi.org/10.1117/12.2561253
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KEYWORDS
Chemical analysis
Chromatography
Luminescence
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