Assessment of cell death using dynamic light scattering with M-mode optical coherence tomography imaging

Sehar Rija; Michael C. Kolios

doi:10.1117/12.2545364

21 February 2020 Assessment of cell death using dynamic light scattering with M-mode optical coherence tomography imaging

Sehar Rija, Michael C. Kolios

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Proceedings Volume 11253, Biomedical Applications of Light Scattering X; 112530Q (2020) https://doi.org/10.1117/12.2545364
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Optical Coherence tomography (OCT) is a rapidly developing light based imaging modality that can provide functional information and visualization of tissue morphology and cell death by quantifying the motion of intracellular structures. In this work, Dynamic light scattering (DLS) with OCT, is used to compute the time-dependent fluctuations in scattered light intensity. Using the DLS-OCT method, we have previously shown a higher rate of intracellular motion detected in apoptotic cells compared to viable cells. In this study, we aim to probe the intracellular motion of cells at much higher scan-rates that we have attempted previously to detect sub-cellular motion with greater sensitivity. To validate the DLS-OCT approach, M-mode OCT images were acquired using Thorlabs SS-OCT system (sampling rate of 100 kHz) to measure the Brownian motion in monodisperse microsphere suspensions. The results demonstrate that ACF decays more rapidly for smaller size microspheres and the experimental decorrelation time values matched with the theoretical values calculated using the Einstein-Stokes equation.

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Sehar Rija and Michael C. Kolios "Assessment of cell death using dynamic light scattering with M-mode optical coherence tomography imaging", Proc. SPIE 11253, Biomedical Applications of Light Scattering X, 112530Q (21 February 2020); https://doi.org/10.1117/12.2545364

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KEYWORDS

Optical coherence tomography

Cell death

Coherence imaging

Dynamic light scattering

Motion measurement

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