Using quantitative phase imaging to acquire angular scattering information from single macrophages (Conference Presentation)

Robert L. Draham; Kaitlin J. Dunn; Elizabeth A. M. Lister; Jonathan J. Pinney; Michael R. Elliott; Andrew J. Berger

doi:10.1117/12.2542684

10 March 2020 Using quantitative phase imaging to acquire angular scattering information from single macrophages (Conference Presentation)

Robert L. Draham, Kaitlin J. Dunn, Elizabeth A. M. Lister, Jonathan J. Pinney, Michael R. Elliott, Andrew J. Berger

Author Affiliations +

Proceedings Volume 11253, Biomedical Applications of Light Scattering X; 112530F (2020) https://doi.org/10.1117/12.2542684
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Angle-resolved light scattering microscopy enables the size distribution of scatterers within a cell to be estimated non-invasively. Our group is working towards obtaining quantitative estimates of mean scatterer size in single cells by comparing the angular distribution of scattered light to Mie theory models. Using quantitative phase imaging, we are able to measure multiple cells at a time and still obtain each cell’s scattering pattern individually. We are now acquiring data from macrophages performing antibody-dependent cellular phagocytosis (ADCP) in hopes of relating the size estimates to the different known stages of the process.

Conference Presentation

Citation Download Citation

Robert L. Draham, Kaitlin J. Dunn, Elizabeth A. M. Lister, Jonathan J. Pinney, Michael R. Elliott, and Andrew J. Berger "Using quantitative phase imaging to acquire angular scattering information from single macrophages (Conference Presentation)", Proc. SPIE 11253, Biomedical Applications of Light Scattering X, 112530F (10 March 2020); https://doi.org/10.1117/12.2542684

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