In vivo chemical alteration profiling Caenorhabditis elegans during development and aging by hyperspectral stimulated Raman scattering microscopy (Conference Presentation)

Tao Chen; Dinghuan Deng; Ayse Sena Mutlu; Yong Yu; Meng C. Wang

doi:10.1117/12.2549054

10 March 2020 In vivo chemical alteration profiling Caenorhabditis elegans during development and aging by hyperspectral stimulated Raman scattering microscopy (Conference Presentation)

Tao Chen, Dinghuan Deng, Ayse Sena Mutlu, Yong Yu, Meng C. Wang

Author Affiliations +

Proceedings Volume 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine; 1125206 (2020) https://doi.org/10.1117/12.2549054
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We systematically investigate the changes of lipid and protein contents at all developmental stages of Caenorhabditis elegans, including L1 to L4 larvae, and adults, using the recently developed hyperspectral stimulated Raman scattering (hsSRS) microscopy. The gut granules in larvae, which are known as lysosome-like organelles, are specifically identified and the temporal change of these gut granules are tracked at different stages. By acquiring the spectral images of C. elegans at different ages and analyzing the lipid spectra change statistically with hsSRS, we will foster a better understanding of the physiological and pathophysiological processes in living organisms.

Conference Presentation

Citation Download Citation

Tao Chen, Dinghuan Deng, Ayse Sena Mutlu, Yong Yu, and Meng C. Wang "In vivo chemical alteration profiling Caenorhabditis elegans during development and aging by hyperspectral stimulated Raman scattering microscopy (Conference Presentation)", Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 1125206 (10 March 2020); https://doi.org/10.1117/12.2549054

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