Synchronized subharmonic modulation in stimulated emission microscopy (Conference Presentation)

Fu-Jen Kao

doi:10.1117/12.2544936

9 March 2020 Synchronized subharmonic modulation in stimulated emission microscopy (Conference Presentation)

Fu-Jen Kao

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 112441E (2020) https://doi.org/10.1117/12.2544936
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

In this work, we have demonstrated a stimulated emission (SE) based pump-probe microscopy with subharmonic modulation and synchronization on the pump and the probe lasers. Critically, a high frequency divider circuit divides the repetition frequency (76 MHz) of the probe laser (Ti:sapphire) to the half repetition frequency (38 MHz), which in turn drives the pump laser (pulsed gain-switched diode laser) synchronously and provides the reference signal for lock-in detection. In this way, the highest possible modulation frequency can be achieved for lock-in detection with shot noise limited sensitivity. The greatly shortened time constant (< 0.1 ms) further improved imaging speed. Over an order of magnitude improvement in signal-to-noise ratio is achieved, when compared with conventional lock-in detection implement.

Conference Presentation

Citation Download Citation

Fu-Jen Kao "Synchronized subharmonic modulation in stimulated emission microscopy (Conference Presentation)", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441E (9 March 2020); https://doi.org/10.1117/12.2544936

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