OCT analysis of mouse reproduction and early development in vivo: dynamics of cilia, eggs and sperm (Conference Presentation)

Irina V. Larina

doi:10.1117/12.2555061

9 March 2020 OCT analysis of mouse reproduction and early development in vivo: dynamics of cilia, eggs and sperm (Conference Presentation)

Irina V. Larina

Author Affiliations +

Proceedings Volume 11239, Dynamics and Fluctuations in Biomedical Photonics XVII; 112390U (2020) https://doi.org/10.1117/12.2555061
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We recently established a set of unique in vivo methods for investigation of early embryonic development and reproduction in mice. Without the use of any contrast agents, these methods allow for live dynamic volumetric imaging of mouse reproductive tract with micro-scale spatial resolution using optical coherence tomography (OCT), in vivo depth-resolved mapping of the cilia location and cilia beat frequency (CBF) in the Fallopian tube (oviduct), and tracking individual sperm and their motility in vivo in the oviduct. Our observations revealed intriguing findings, which question current views on role of cilia in gamete and embryo transfer during preimplantation pregnancy.

Conference Presentation

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Irina V. Larina "OCT analysis of mouse reproduction and early development in vivo: dynamics of cilia, eggs and sperm (Conference Presentation)", Proc. SPIE 11239, Dynamics and Fluctuations in Biomedical Photonics XVII, 112390U (9 March 2020); https://doi.org/10.1117/12.2555061

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