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Most biosensors rely on immobilized antibodies or aptamers. In contrast, receptor proteins exist naturally in lipid bilayers and are highly specific to small molecules. We use a frequency-locked optical whispering evanescent resonator (FLOWER) system for real-time quantification of rhodopsin incorporation into an artificial lipid membrane and observe photo-induced molecular transformations upon light activation. Our study of proteolipid membrane coated microtoroids for probing the local activity of G-protein coupled receptors was further expanded to kappa-opioid receptors and their endogenous ligand Dynorphin-A. G-protein coupled receptor signaling probed by a microtoroid-proteolipid system will facilitate drug discovery and therapeutic interventions.
Phuong Diem Nguyen,Adley Gin,Cheng Li, andJudith Su
"G-protein coupled receptor signaling probed by a microtoroid-proteolipid system (Conference Presentation)", Proc. SPIE 11233, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XX, 112330M (9 March 2020); https://doi.org/10.1117/12.2545495
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Phuong Diem Nguyen, Adley Gin, Cheng Li, Judith Su, "G-protein coupled receptor signaling probed by a microtoroid-proteolipid system (Conference Presentation)," Proc. SPIE 11233, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XX, 112330M (9 March 2020); https://doi.org/10.1117/12.2545495