Multiphoton imaging of normal breast lobules, pleomorphic invasive lobular carcinoma, and classic invasive lobular carcinoma

Jiajia He; Tingfeng Shen; Deyong Kang; Shu Wang; Wenhui Guo; Lianhuang Li; Liqin Zheng; Chuan Wang; Jianxin Chen

doi:10.1117/12.2537547

20 November 2019 Multiphoton imaging of normal breast lobules, pleomorphic invasive lobular carcinoma, and classic invasive lobular carcinoma

Jiajia He, Tingfeng Shen, Deyong Kang, Shu Wang, Wenhui Guo, Lianhuang Li, Liqin Zheng, Chuan Wang, Jianxin Chen

Author Affiliations +

Proceedings Volume 11190, Optics in Health Care and Biomedical Optics IX; 111902O (2019) https://doi.org/10.1117/12.2537547
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

Pleomorphic invasive lobular carcinoma (pILC) is a distinct morphological variant of invasive lobular carcinoma (ILC), which is more aggressive than classic invasive lobular carcinoma (cILC) with worse overall prognosis. It is critical to distinguish between pILC and cILC for management and treatment of patients. Multiphoton microscopy (MPM),based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG), produces subcellular resolution images to show detailed information on structure and cell morphology of tissue sections without staining. In this work, MPM was introduced for label-free identification of normal breast lobules, pILC and cILC, which is consistent with haematoxylin and eosin (H&E) staining. Furthermore, analysis of nuclear area is used to quantitatively reflect their cytological characteristic. These results demonstrate that, MPM has the ability to distinguish between normal breast lobules, cILC, and pILC by histopathological features, which can provide additional indicators to determine adequate treatment strategies of patients with pILC for pathologists and surgeons. In the future, MPM may be used as a diagnostic tool of lobular cancer into routine clinical use.

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Jiajia He, Tingfeng Shen, Deyong Kang, Shu Wang, Wenhui Guo, Lianhuang Li, Liqin Zheng, Chuan Wang, and Jianxin Chen "Multiphoton imaging of normal breast lobules, pleomorphic invasive lobular carcinoma, and classic invasive lobular carcinoma", Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111902O (20 November 2019); https://doi.org/10.1117/12.2537547

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