Single-agent liposomal chemophototherapy for rat mammary tumors (Conference Presentation)

Jonathan Lovell

doi:10.1117/12.2526214

14 August 2019 Single-agent liposomal chemophototherapy for rat mammary tumors (Conference Presentation)

Jonathan Lovell

Proceedings Volume 11070, 17th International Photodynamic Association World Congress; 1107043 (2019) https://doi.org/10.1117/12.2526214
Event: 17th International Photodynamic Association World Congress, 2019, Cambridge, Massachusetts, United States

Abstract

Photosensitizers can be integrated with drug delivery vehicles to develop chemophototherapy agents with anti-tumor synergy between chemo- and photo- components. Long-circulating doxorubicin (Dox) in porphyrin-phospholipid (PoP) liposomes (LC-Dox-PoP) incorporates a phospholipid-like photosensitizer (2 mole %) in the bilayer of Dox-loaded stealth liposomes. Hematological effects of endotoxin-minimized LC-Dox-PoP were characterized via standardized assays. In vitro interaction with erythrocytes, platelets, and plasma coagulation cascade were generally unremarkable while complement activation was found to be similar to that of commercial Doxil. Blood partitioning suggested both the Dox and PoP components of LC-Dox-PoP were stably entrapped or incorporated in liposomes. This was further confirmed with pharmacokinetic studies in Fischer rats, which showed the PoP and Dox components of the liposomes both had nearly identical, long circulation half-lives (25-26 hours). In a large orthotopic mammary tumor model in Fischer rats, following intravenous dosing (2 mg/kg Dox), the depth of enhanced Dox delivery in response to 665 nm laser irradiation was over 1 cm. LC-Dox-PoP with laser treatment cured or potently suppressed tumor growth, with greater efficacy observed in tumors 0.8-1.2 cm compared to larger ones. The skin at the treatment site healed within approximately 30 days. Taken together, these data provide insight into nanocharacterization and photo-ablation parameters for a chemophototherapy agent.

Conference Presentation

Citation Download Citation

Jonathan Lovell "Single-agent liposomal chemophototherapy for rat mammary tumors (Conference Presentation)", Proc. SPIE 11070, 17th International Photodynamic Association World Congress, 1107043 (14 August 2019); https://doi.org/10.1117/12.2526214

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