Exploiting cellular delivery of conjugated polymer nanoparticles for improved photodynamic therapy in a 3D glioblastoma model

L. E. Ibarra; L. Beaugé; C. A. Chesta; V. A. Rivarola; R. E. Palacios

doi:10.1117/12.2526763

7 August 2019 Exploiting cellular delivery of conjugated polymer nanoparticles for improved photodynamic therapy in a 3D glioblastoma model

L. E. Ibarra, L. Beaugé, C. A. Chesta, V. A. Rivarola, R. E. Palacios

Author Affiliations +

Proceedings Volume 11070, 17th International Photodynamic Association World Congress; 1107012 (2019) https://doi.org/10.1117/12.2526763
Event: 17th International Photodynamic Association World Congress, 2019, Cambridge, Massachusetts, United States

Abstract

Photodynamic Therapy (PDT) has recently gain attention as alternative treatment of Central Nervous System (CNS) cancer diseases, due to the demonstration of successfully elimination of gliomas in patients. The implementation of PDT for brain tumors, and especially glioblastoma (GBM), has already been approved in some countries. Due to their superb light absorption and photostability conjugated polymer nanoparticles (CPNs) are promising photosensitizers (PS) for use in PDT. Recently, we developed metallated porphyrin-doped CPNs for PDT and demonstrated that they were effective eliminating glioma cells trough ROS-mediated photoinduced damage. A problem of many therapies used to eradicate brain gliomas is the difficulty of arrival and preferential accumulation of the active drug into the tumor upon systemic administration due to the selective permeability of the blood-brain barrier (BBB). To solve this problem our approach employs mononuclear cells, which can cross BBB and infiltrate tumors, as stealth carriers for drug delivery into brain tumors. In this study loading of CPNs into monocytes/macrophages was demonstrated and the cellular functionality, chemotaxis and penetration of these loaded monocytes/macrophages into GBM spheroids (3D tumor models) was tested. CPNs loading was successfully achieved using human monocytes THP-1 and mouse bone marrow-derived monocytes (BMdM) without disturbing cell viability and differentiation potential towards macrophage state. CPNs-loaded monocytes were found to better infiltrate spheroids as compared to CPNs. Furthermore, PDT efficacy on GBM spheroids was improved when using our monocyte-mediated delivery strategy

Conference Presentation

Citation Download Citation

L. E. Ibarra, L. Beaugé, C. A. Chesta, V. A. Rivarola, and R. E. Palacios "Exploiting cellular delivery of conjugated polymer nanoparticles for improved photodynamic therapy in a 3D glioblastoma model", Proc. SPIE 11070, 17th International Photodynamic Association World Congress, 1107012 (7 August 2019); https://doi.org/10.1117/12.2526763

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available