CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 11067 > Article
Paper
3 June 2019 Spectrophotometric vs. colorimetric analysis of mycobacterium tuberculosis population growth curves in resazurin assay
Eugene B. Postnikov, Anastasia I. Lavrova, Andrey A. Khalin, Marine Z. Dogonadze, Olga A. Manicheva
Author Affiliations +
Proceedings Volume 11067, Saratov Fall Meeting 2018: Computations and Data Analysis: from Nanoscale Tools to Brain Functions; 110670L (2019) https://doi.org/10.1117/12.2523300
Event: International Symposium on Optics and Biophotonics VI: Saratov Fall Meeting 2018, 2018, Saratov, Russian Federation
Abstract
The rezazurin assay for screening of bacterial growth remains the most popular method for express diagnostic tests. It is based on the change of colour and uorescence properties of the respective dye due to bacterial breathing but its conventional quantitative practical implementation requires a relative expensive and cumbersome spectrophotometric equipment. As an alternative approach, we propose to shift a focus from hardware to software. Preliminary results announced in this work show that the processing RGB colour channels for simple photographic images of resazurin assay plates with mycobacterium tuberculosis provides an opportunity to reconstruct the target growth curve with an accuracy comparable with results obtained using the conventional photometric uorescence curves.
© (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Eugene B. Postnikov, Anastasia I. Lavrova, Andrey A. Khalin, Marine Z. Dogonadze, and Olga A. Manicheva "Spectrophotometric vs. colorimetric analysis of mycobacterium tuberculosis population growth curves in resazurin assay", Proc. SPIE 11067, Saratov Fall Meeting 2018: Computations and Data Analysis: from Nanoscale Tools to Brain Functions, 110670L (3 June 2019); https://doi.org/10.1117/12.2523300
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 7 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Visualization
Analytical research
Cameras
Photography
RGB color model
Spectrophotometry
Microorganisms
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top