Automatized fine-focusing in digital microscopy

Petr Pokorný; Filip Šmejkal; Pavel Novák; Jirí Novák; Antonín Mikš

doi:10.1117/12.2525421

21 June 2019 Automatized fine-focusing in digital microscopy

Petr Pokorný, Filip Šmejkal, Pavel Novák, Jirí Novák, Antonín Mikš

Author Affiliations +

Proceedings Volume 11062, Digital Optical Technologies 2019; 110621I (2019) https://doi.org/10.1117/12.2525421
Event: SPIE Digital Optical Technologies, 2019, Munich, Germany

Abstract

This paper proposes a procedure for automatized fine-focusing of particles’ image in digital microscopy. First, analytical formulas for a calculation of axial defocus are derived using the complex amplitude propagation. Afterwards, the formulas are used for a design of an iterative algorithm for the fine-focusing. Uncertainty analysis and error simulations are then presented. In the final part of the paper, the method is verified by a simple experimental setup. The proposed strategy can find utilisation in many practical applications of automatized processes, not only in digital microscopy.

Citation Download Citation

Petr Pokorný, Filip Šmejkal, Pavel Novák, Jirí Novák, and Antonín Mikš "Automatized fine-focusing in digital microscopy", Proc. SPIE 11062, Digital Optical Technologies 2019, 110621I (21 June 2019); https://doi.org/10.1117/12.2525421

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