Using multiphoton microscopy to assess pulmonary emphysema in mouse models

Liqin Zheng; Yina Huang; Zhida Chen; Xiaomin Huang; Yixuan Chen; Jianxin Chen

doi:10.1117/12.2506331

15 November 2018 Using multiphoton microscopy to assess pulmonary emphysema in mouse models

Liqin Zheng, Yina Huang, Zhida Chen, Xiaomin Huang, Yixuan Chen, Jianxin Chen

Proceedings Volume 10964, Tenth International Conference on Information Optics and Photonics; 109645C (2018) https://doi.org/10.1117/12.2506331
Event: Tenth International Conference on Information Optics and Photonics (CIOP 2018), 2018, Beijing, China

Abstract

Chronic obstructive pulmonary diseases (COPD) is the fifth leading cause of death worldwide and will be increased in the coming decades. Pulmonary emphysema is one of the hallmarks of COPD. Establishing the animal model of pulmonary emphysema is very important to explore its pathogenesis. Until now, researchers are still having used histological methods to assess whether it is successful to stimulate the emphysema animal model. In this study, we try to use multiphoton microscopy imaging system to assess whether the mouse models obtained the emphysema pathological. The two-photon excited fluorescence (TPEF) signals and second harmonic generation (SHG) signals clearly showed changes in both cellular features and extracellular matrix architecture during different time of emphysema mouse models. With the development of miniaturized multiphoton microscopy, multiphoton microscopy can be used to monitor the developing of pulmonary emphysema in animals in vivo.

Citation Download Citation

Liqin Zheng, Yina Huang, Zhida Chen, Xiaomin Huang, Yixuan Chen, and Jianxin Chen "Using multiphoton microscopy to assess pulmonary emphysema in mouse models", Proc. SPIE 10964, Tenth International Conference on Information Optics and Photonics, 109645C (15 November 2018); https://doi.org/10.1117/12.2506331

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KEYWORDS

Emphysema

Multiphoton microscopy

Chronic obstructive pulmonary disease

Tissues

Animal model studies

Imaging systems

Second-harmonic generation

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