Holographic scanning microscope: simulation of hologram recording and wavefront reconstruction (Conference Presentation)

Maria Muravyeva; Yuri Zakharov

doi:10.1117/12.2508926

13 March 2019 Holographic scanning microscope: simulation of hologram recording and wavefront reconstruction (Conference Presentation)

Maria Muravyeva, Yuri Zakharov

Author Affiliations +

Proceedings Volume 10944, Practical Holography XXXIII: Displays, Materials, and Applications; 109440K (2019) https://doi.org/10.1117/12.2508926
Event: SPIE OPTO, 2019, San Francisco, California, United States

Abstract

We have constructed a Holographic Scanning Microscopy (HSM) setup, which employs an optical design and a scanning unit of a commercial confocal laser scanning microscope. This arrangement helps to construct the HSM easier, but the scanning unit gives additional phase distortions modifying an object wave and introducing phase shift. We offer a way to dispose such distortions changing the way of recording holograms and reconstruction algorithm. Analyzing recorded holograms we conclude, that we need to record a hologram of flat mirror first, which would contain required information about phase distortions, and use it for reconstruction. Finally reconstruction algorithm becomes like to “coded” reference wave reconstruction algorithm. Proposed algorithm was successfully implemented and tested in MatLab.

Conference Presentation

Citation Download Citation

Maria Muravyeva and Yuri Zakharov "Holographic scanning microscope: simulation of hologram recording and wavefront reconstruction (Conference Presentation)", Proc. SPIE 10944, Practical Holography XXXIII: Displays, Materials, and Applications, 109440K (13 March 2019); https://doi.org/10.1117/12.2508926

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