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7 March 2019 Improving the sensitivity of fluorescence-based immunoassays by photobleaching the autofluorescence of magnetic beads
Shira Roth, Orr Hadass, Meir Cohen, Jasenka Verbarg, Jennifer Wilsey, Amos Danielli
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Proceedings Volume 10895, Frontiers in Biological Detection: From Nanosensors to Systems XI; 1089509 (2019) https://doi.org/10.1117/12.2507597
Event: SPIE BiOS, 2019, San Francisco, California, United States
Abstract
One of the most common capture surfaces is a magnetic bead. However, magnetic beads exhibit strong autofluorescence, which often overlaps with the emission of the reporter fluorescent dyes and limits the analytical sensitivity of the assay. Here, we photobleached several widely used magnetic beads and reduced their autofluorescence to 1% of the initial value. The photobleached beads were stable over time and their surface functionality was retained. In a high sensitivity LX-200™ system using photobleached magnetic beads, human interleukin-8 was detected with a 3-fold improvement in analytical sensitivity and signal to noise ratio over results achievable with non-bleached beads.
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Shira Roth, Orr Hadass, Meir Cohen, Jasenka Verbarg, Jennifer Wilsey, and Amos Danielli "Improving the sensitivity of fluorescence-based immunoassays by photobleaching the autofluorescence of magnetic beads", Proc. SPIE 10895, Frontiers in Biological Detection: From Nanosensors to Systems XI, 1089509 (7 March 2019); https://doi.org/10.1117/12.2507597
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KEYWORDS
Magnetism
Signal to noise ratio
Biosensing
Signal detection
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