CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 10892 > Article
Presentation
13 March 2019 A gold nanoparticle bio-optical transponder to dynamically monitor intracellular events (Conference Presentation)
Geoffrey F. Strouse
Author Affiliations +
Proceedings Volume 10892, Colloidal Nanoparticles for Biomedical Applications XIV; 108920V (2019) https://doi.org/10.1117/12.2510456
Event: SPIE BiOS, 2019, San Francisco, California, United States
Abstract
The coupling of a gold nanoparticle to peptides and/or nucleic acids can allow imaging of intracellular events when optically labelled by molecular dyes. The use of a gold bio-optical transponder (BOT) capable of simultaneously reporting the timing of intracellular cargo delivery, cargo release from a gold nanoparticle, and subsequent cellular processing provides direct insight into the endo-lysosomal processing of nano-based delivery probes. The paper will explore use of BOT sensors to monitor peptide dependent uptake of AuNPs into native vs drug resistant melanoma cells; monitor the effect off surface coupling of gene knock-in, and explore the ability to evaluate the evolving intracellular pH (pHi) during endosomal maturation. The BOT is designed around use of multicolor surface energy transfer (SET) to simultaneously track processes and provide an internal reference for ratiometric analysis of events. The use of a BOT based sensor to explore intracellular processing may enable greater insight into our understanding of the fundamental processes of biology.
Conference Presentation
© (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Geoffrey F. Strouse "A gold nanoparticle bio-optical transponder to dynamically monitor intracellular events (Conference Presentation)", Proc. SPIE 10892, Colloidal Nanoparticles for Biomedical Applications XIV, 108920V (13 March 2019); https://doi.org/10.1117/12.2510456
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Gold
Nanoparticles
Transponders
Sensors
Biology
Energy transfer
Melanoma
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top