CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 10890 > Article
Presentation
4 March 2019 Label free detection of pre-cancerous cells in vivo with a/LCI (Conference Presentation)
Adam Wax
Author Affiliations +
Proceedings Volume 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019; 1089014 (2019) https://doi.org/10.1117/12.2507063
Event: SPIE BiOS, 2019, San Francisco, California, United States
Abstract
Detection of pre-cancerous (dysplastic) tissues in the clinic remains an ongoing challenge. Current methods often rely on systematic biopsies with no visual cues to guide the physician to areas likely to harbor dysplasia. We have developed novel spectroscopic techniques for assessing cell structure and diagnosing disease based on combining interferometry with light scattering. Angle-resolved low coherence interferometry (a/LCI) combines the depth gating of coherence imaging, as used in optical coherence tomography, with the high resolution structural information that can be obtained with light scattering spectroscopy. The a/LCI approach has been validated with in vitro measurements of phantoms and in vitro cells to recover sub-cellular structure with sub-wavelength accuracy and precision. Discrimination of pre-cancerous tissue states was initially demonstrated using animal models and ex vivo human tissues, by using light scattering and interferometry to measure the size of cell nuclei in selected tissue layers. We will discuss various light scattering models used in a/LCI, including Mie theory, T-matrix, and analysis methods based on wavelet transforms and machine learning, as they relate to their application to this clinical problem. To implement a/LCI for detection of pre-cancerous tissues in patients, we have developed a clinical a/LCI system, including a portable optical engine, and an endoscope compatible fiber probe for in vivo measurements. The capabilities of a/LCI will be shown with results from clinical studies to detect precancerous cells in both the esophagus and cervix during routine clinical examination. Further directions to enhance clinical utility and ergonomic ease of use will also be discussed.
Conference Presentation
© (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Adam Wax "Label free detection of pre-cancerous cells in vivo with a/LCI (Conference Presentation)", Proc. SPIE 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019, 1089014 (4 March 2019); https://doi.org/10.1117/12.2507063
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
In vivo imaging
Tissues
Light scattering
Interferometry
Animal model studies
In vitro testing
Spectroscopy
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top