Hyperspectral phase imaging with a spatially matched acousto-optical tunable filter

Konstantin B. Yushkov; Justine Champagne; Jean-Claude Kastelik; Vladimir Y. Molchanov

doi:10.1117/12.2503780

4 March 2019 Hyperspectral phase imaging with a spatially matched acousto-optical tunable filter

Konstantin B. Yushkov, Justine Champagne, Jean-Claude Kastelik, Vladimir Y. Molchanov

Proceedings Volume 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019; 108900V (2019) https://doi.org/10.1117/12.2503780
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

We report a novel concept of an advanced hyperspectral imaging system based on acousto-optical tunable filters (AOTFs) with optional phase imaging modality. Visualization of phase objects is performed using an additional adjustable liquid crystal amplitude mask. The mask shape is matched with the two-dimensional transfer function of the AOTF enabling bandpass spatial filtering. Label-free phase object visualization is demonstrated with unstained histological sections using diascopic incoherent illumination of a standard inverted light microscope.

Conference Presentation

Citation Download Citation

Konstantin B. Yushkov, Justine Champagne, Jean-Claude Kastelik, and Vladimir Y. Molchanov "Hyperspectral phase imaging with a spatially matched acousto-optical tunable filter ", Proc. SPIE 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019, 108900V (4 March 2019); https://doi.org/10.1117/12.2503780

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