Paper
24 April 2018 Development of dual-wavelength microscopic laser speckle contrast imaging system
Author Affiliations +
Abstract
Laser Speckle Contrast Imaging (LSCI), which used coherent light, has fully been used for observing blood flow due to its non-invasive, non-contact acquisition method. Generally, LSCI system uses just a single wavelength for measurement. In this research, first, considering the biological characteristics of different reflection rates and absorption, we use two lasers at 633nm and 855 nm and two CCD cameras to build a microscopic LSCI system. Second, by using Spatial, Temporal Speckle Contrast Analysis methods and analysis with Beer–Lambert law, the microcirculation can be in vivo visualized and oxygenation can be observed. Such developed system can be further used for in vivo animal studies.
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Cheng-Yu Lee and Meng-Tsan Tsai "Development of dual-wavelength microscopic laser speckle contrast imaging system", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107111G (24 April 2018); https://doi.org/10.1117/12.2317069
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Speckle

Laser speckle contrast imaging

Imaging systems

CCD cameras

Image analysis

Light sources

Absorption

Back to Top