Coherent brightfield (COBRI) microscopy for ultrahigh-speed single-particle tracking on lipid bilayer membranes

Yi-Hung Liao; Chia-Lung Hsieh

doi:10.1117/12.2319565

24 April 2018 Coherent brightfield (COBRI) microscopy for ultrahigh-speed single-particle tracking on lipid bilayer membranes

Yi-Hung Liao, Chia-Lung Hsieh

Author Affiliations +

Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 1071105 (2018) https://doi.org/10.1117/12.2319565
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

Following the journey of a single molecule is a powerful tool for studying the structures and functions of biological membranes. To resolve the interplays between individual membrane molecules and their nano-associations, membrane dynamics needs to be measured at the nanoscale with high temporal resolution. Here, we demonstrate ultrahigh-speed coherent brightfield (COBRI) microscopy for capturing diffusive motion of single lipid molecules in model membranes labeled by single 40 nm gold nanoparticles with a spatial precision of 3.6 nm and a temporal resolution of 2.56 microseconds (corresponding to a frame rate of 390 kHz) under an illumination intensity of 15 μW/μm2. Our method is readily applicable to the future study of plasma membrane of live cells where the transient confinements in membrane nanodomains could be investigated with unprecedented clarity.

Citation Download Citation

Yi-Hung Liao and Chia-Lung Hsieh "Coherent brightfield (COBRI) microscopy for ultrahigh-speed single-particle tracking on lipid bilayer membranes", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 1071105 (24 April 2018); https://doi.org/10.1117/12.2319565

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