Real-time mapping of cytokine gradients photonic resonant imaging technology (Conference Presentation)

José Juan-Colás; Steven Johnson; Thomas F. Krauss

doi:10.1117/12.2286853

14 March 2018 Real-time mapping of cytokine gradients photonic resonant imaging technology (Conference Presentation)

José Juan-Colás, Steven Johnson, Thomas F. Krauss

Proceedings Volume 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI; 104971C (2018) https://doi.org/10.1117/12.2286853
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Cytokine diffusion and dynamics are central to cellular signaling, activation and migration, yet few, if any, technologies are currently able to quantify cytokine protein behavior in real-time, in a label-free fashion and, most importantly, with a spatial resolution that allows pinpointing individual cells. Photonic crystal resonant imaging offers high spatial and temporal resolution so it is an ideal platform for studying this mammalian extracellular process. Unlike other photonic biosensors, the resonant imaging technique offers very high spatial resolution (of order of a few μm), so it can be understood as an imaging array of highly sensitive biosensors. Here we propose and demonstrate a method of quantifying the secreted chemokine gradients from individual cells. Two Baby Hamster Kidney fibroblast cells lines (i.e. BHK cells) were employed in our studies, engineered to overexpress the chemokine interleukin 3 (IL-3) and the human thrombopoietin growth factor hormone (HTPO), respectively. Localized free cytokine/hormone levels were quantified permitting formation of real-time maps of localized secreted protein levels. The technology has been proven to be protein-specific, greatly minimizing non-specific binding from other carbohydrates secreted from the cells and therefore improving the signal-to-noise ratio of the maps. Ultimately, this system allows the identification of specific cells from mixed systems where diverse types of cells are present. This technique represents a powerful tool to noninvasively and nondestructively study cell behavior in-vitro, label-free, and in real-time; overcoming the inherent challenges of label-based methods.

Conference Presentation

Citation Download Citation

José Juan-Colás, Steven Johnson, and Thomas F. Krauss "Real-time mapping of cytokine gradients photonic resonant imaging technology (Conference Presentation)", Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 104971C (14 March 2018); https://doi.org/10.1117/12.2286853

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Biosensors

Photonic crystals

Proteins

Spatial resolution

Biomedical optics

Diffusion

Imaging arrays

Show All Keywords

Keywords/Phrases

Search In:

Publication Years