Dual-wavelength OR-PAM with compressed sensing for cell tracking in a 3D cell culture system

Rou-Xuan Huang; Ying Fu; Wang Liu; Yu-Ting Ma; Bao-Yu Hsieh; Shu-Ching Chen; Mingjian Sun; Pai-Chi Li

doi:10.1117/12.2289975

19 February 2018 Dual-wavelength OR-PAM with compressed sensing for cell tracking in a 3D cell culture system

Rou-Xuan Huang, Ying Fu, Wang Liu, Yu-Ting Ma, Bao-Yu Hsieh, Shu-Ching Chen, Mingjian Sun, Pai-Chi Li

Author Affiliations +

Proceedings Volume 10494, Photons Plus Ultrasound: Imaging and Sensing 2018; 1049462 (2018) https://doi.org/10.1117/12.2289975
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Monitoring dynamic interactions of T cells migrating toward tumor is beneficial to understand how cancer immunotherapy works. Optical-resolution photoacoustic microscope (OR-PAM) can provide not only high spatial resolution but also deeper penetration than conventional optical microscopy. With the aid of exogenous contrast agents, the dual-wavelength OR-PAM can be applied to map the distribution of CD8+ cytotoxic T lymphocytes (CTLs) with gold nanospheres (AuNS) under 523nm laser irradiation and Hepta1-6 tumor spheres with indocyanine green (ICG) under 800nm irradiation. However, at 1K laser PRF, it takes approximately 20 minutes to obtain a full sample volume of 160 × 160 × 150 μm³ . To increase the imaging rate, we propose a random non-uniform sparse sampling mechanism to achieve fast sparse photoacoustic data acquisition. The image recovery process is formulated as a low-rank matrix recovery (LRMR) based on compressed sensing (CS) theory. We show that it could be stably recovered via nuclear-norm minimization optimization problem to maintain image quality from a significantly fewer measurement. In this study, we use the dual-wavelength OR-PAM with CS to visualize T cell trafficking in a 3D culture system with higher temporal resolution. Data acquisition time is reduced by 40% in such sample volume where sampling density is 0.5. The imaging system reveals the potential to understand the dynamic cellular process for preclinical screening of anti-cancer drugs.

Citation Download Citation

Rou-Xuan Huang, Ying Fu, Wang Liu, Yu-Ting Ma, Bao-Yu Hsieh, Shu-Ching Chen, Mingjian Sun, and Pai-Chi Li "Dual-wavelength OR-PAM with compressed sensing for cell tracking in a 3D cell culture system", Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 1049462 (19 February 2018); https://doi.org/10.1117/12.2289975

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE