LED photochemotherapy against Staphylococcus aureus: an in vitro study

Susana C. P. S. de Oliveira D.D.S.; Juliana S. C. Monteiro; Gustavo M. Pires-Santos; Fernando José Pires Sampaio; Luiz Guilherme P. Soares; Amanda P. Soares; Antônio L. B. Pinheiro

doi:10.1117/12.2286935

8 February 2018 LED photochemotherapy against Staphylococcus aureus: an in vitro study

Susana C. P. S. de Oliveira D.D.S., Juliana S. C. Monteiro, Gustavo M. Pires-Santos, Fernando José Pires Sampaio, Luiz Guilherme P. Soares, Amanda P. Soares, Antônio L. B. Pinheiro

Author Affiliations +

Proceedings Volume 10477, Mechanisms of Photobiomodulation Therapy XIII; 104770S (2018) https://doi.org/10.1117/12.2286935
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Bacterial resistance to antibiotics is reality and need for alternative treatments is urgent. The aim of this work was to evaluate, in vitro, the effect of LED photochemotherapy on Staphylococcus aureus (ATCC 23529) using 25 μg / mL of phenothiazine compound combined with LED light (λ632 ± 2ηm) using 12 J/cm² energy density. The experiments were carried out in triplicate and the samples were divided into groups: Control, Irradiated (treated only with light), Photosensitizer (treated only in the presence of the dye), LED-Photochemotherapy (treatment with light associated with dye). Counts of the colony forming units and the data obtained were statistically analyzed (ANOVA, Tukey’s test, p<0.05). The present study demonstrated that the efficacy of LED-Photochemotherapy as the use of 25 μg/mL x 12 J/cm² caused 91.57 % of inhibition of bacterial growth. It is concluded that using energy density of 12 J/cm² associated to 25 μg/mL caused high in vitro inhibition of S. aureus.

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Susana C. P. S. de Oliveira D.D.S., Juliana S. C. Monteiro, Gustavo M. Pires-Santos, Fernando José Pires Sampaio, Luiz Guilherme P. Soares, Amanda P. Soares, and Antônio L. B. Pinheiro "LED photochemotherapy against Staphylococcus aureus: an in vitro study ", Proc. SPIE 10477, Mechanisms of Photobiomodulation Therapy XIII, 104770S (8 February 2018); https://doi.org/10.1117/12.2286935

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