Dual-channel (green and red) fluorescence microendoscope with subcellular resolution

Camila de Paula D'Almeida; Thereza Cury Fortunato; Ramon Gabriel Teixeira Rosa; Renan Arnon Romano; Lilian Tan Moriyama; Sebastião Pratavieira

doi:10.1117/12.2290923

14 February 2018 Dual-channel (green and red) fluorescence microendoscope with subcellular resolution

Camila de Paula D'Almeida, Thereza Cury Fortunato, Ramon Gabriel Teixeira Rosa, Renan Arnon Romano, Lilian Tan Moriyama, Sebastião Pratavieira

Author Affiliations +

Proceedings Volume 10470, Endoscopic Microscopy XIII; 1047016 (2018) https://doi.org/10.1117/12.2290923
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Usually, tissue images at cellular level need biopsies to be done. Considering this, diagnostic devices, such as microendoscopes, have been developed with the purpose of do not be invasive. This study goal is the development of a dual-channel microendoscope, using two fluorescent labels: proflavine and protoporphyrin IX (PpIX), both approved by Food and Drug Administration. This system, with the potential to perform a microscopic diagnosis and to monitor a photodynamic therapy (PDT) session, uses a halogen lamp and an image fiber bundle to perform subcellular image. Proflavine fluorescence indicates the nuclei of the cell, which is the reference for PpIX localization on image tissue. Preliminary results indicate the efficacy of this optical technique to detect abnormal tissues and to improve the PDT dosimetry. This was the first time, up to our knowledge, that PpIX fluorescence was microscopically observed in vivo, in real time, combined to other fluorescent marker (Proflavine), which allowed to simultaneously observe the spatial localization of the PpIX in the mucosal tissue. We believe this system is very promising tool to monitor PDT in mucosa as it happens. Further experiments have to be performed in order to validate the system for PDT monitoring.

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Citation Download Citation

Camila de Paula D'Almeida, Thereza Cury Fortunato, Ramon Gabriel Teixeira Rosa, Renan Arnon Romano, Lilian Tan Moriyama, and Sebastião Pratavieira "Dual-channel (green and red) fluorescence microendoscope with subcellular resolution", Proc. SPIE 10470, Endoscopic Microscopy XIII, 1047016 (14 February 2018); https://doi.org/10.1117/12.2290923

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