Fluorescence visualization of wound closure in partial and full-thickness wound models (Conference Presentation)

Ying Wang; Antonio Ortega-Martinez; William A. Farinelli; Richard R. Anderson; Walfre Franco

doi:10.1117/12.2287164

14 March 2018 Fluorescence visualization of wound closure in partial and full-thickness wound models (Conference Presentation)

Ying Wang, Antonio Ortega-Martinez, William A. Farinelli, Richard R. Anderson, Walfre Franco

Author Affiliations +

Proceedings Volume 10467, Photonics in Dermatology and Plastic Surgery 2018; 104670P (2018) https://doi.org/10.1117/12.2287164
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Background and Objectives: UV Fluorescence Excitation Imaging (u-FEI) has been shown to be a simple but robust, non-invasive and non-contact method to visualize cells with a high proliferative rate. We had demonstrated the ability of u-FEI to visualize the re-epithelialization of skin wounds in an organ culture system. In this work, we investigated the potential of u-FEI for visualization of wound closure of partial and full-thickness skin wounds. Study Design: Partial and full-thickness skin wounds were created in the tail of rats. Wounds were imaged weekly using u-FEI system operating at 295/340nm excitation/emission wavelengths, which correspond to the excitation/emission bands of the endogenous fluorophore tryptophan. Histology and immunohistology were used to determine the association between fluorescence intensity and proliferation of keratinocyte cells. Results: Similar to human skin, the skin of a rat tail heals by re-epithelialization. Keratinocytes migrated and proliferated from the edge and skin appendages of partial-skin wounds to close the wound by creating neo-epidermis. The fluorescence intensity of the whole wound area increased uniformly during week one and decreased to non-wounded control levels around week three. For full-thickness wounds, keratinocytes migrated only from the wound edges as skin appendages were missing. The fluorescence intensity was higher by the wound edge and marched towards the center during healing. H&E and immunohistology show that changes in fluorescence intensity corresponded to newly formed epidermis. Conclusions: u-FEI of tryptophan allowed visualization of wound closure of partial and full-thickness skin wounds in an in vivo model of wound healing by epithelialization.

Conference Presentation

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Ying Wang, Antonio Ortega-Martinez, William A. Farinelli, Richard R. Anderson, and Walfre Franco "Fluorescence visualization of wound closure in partial and full-thickness wound models (Conference Presentation)", Proc. SPIE 10467, Photonics in Dermatology and Plastic Surgery 2018, 104670P (14 March 2018); https://doi.org/10.1117/12.2287164

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KEYWORDS

Skin

Wound healing

Luminescence

Visualization

Visual process modeling

In vivo imaging

Ultraviolet radiation

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