Paper
18 April 2017 Simultaneous three-dimensional imaging of multi-focal microscopy
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Abstract
High-speed microscopy three-dimensional (3D) microscopy based on trans-illumination is implemented with an amplitude light modulator placed at the Fourier plane of the system. The phase of an incident wave-front is modified and encoded with a defocus parameter to divert the light onto different portion of an image plane depending on their diffraction order and depth positions. The design of the grating pattern for the light modulated is discussed through the simulation and the experiment. 3D imaging capability is demonstrated through the experiment.
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Chen Yen Lin, Yi-Tun Lin, and Yuan Luo "Simultaneous three-dimensional imaging of multi-focal microscopy", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510Y (18 April 2017); https://doi.org/10.1117/12.2269600
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KEYWORDS
3D image processing

Microscopy

Diffraction gratings

Imaging systems

Diffraction

Modulators

3D acquisition

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