Chemometric endogenous fluorescence for tissue diagnosis

Run Li; Kevin Vasquez; M. Xu

doi:10.1117/12.2255615

17 February 2017 Chemometric endogenous fluorescence for tissue diagnosis

Run Li, Kevin Vasquez, M. Xu

Proceedings Volume 10060, Optical Biopsy XV: Toward Real-Time Spectroscopic Imaging and Diagnosis; 1006017 (2017) https://doi.org/10.1117/12.2255615
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Endogenous fluorescence is a powerful technique for probing both structure and function of tissue. We show that enabling wide-field fluorescence microscopy with chemometrics can significantly enhance the performance of tissue diagnosis with endogenous fluorescence. The spatial distribution and absolute concentration of fluorophores is uncovered with non-negative factorization aided by the spatial diversity from microscopic autofluorescence color images. Fluorescence quantification in terms of its absolute concentration map avoids issues dependent on specific measurement approach or systems and yields biologically meaningful data. The standardization of endogenous fluorescence in terms of absolute concentration will facilitate its translation to the clinics and simplifies the assessment of competing methods relating to tissue fluorescence.

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Citation Download Citation

Run Li, Kevin Vasquez, and M. Xu "Chemometric endogenous fluorescence for tissue diagnosis", Proc. SPIE 10060, Optical Biopsy XV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 1006017 (17 February 2017); https://doi.org/10.1117/12.2255615

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