Initial proof-of-concept of photoacoustic cell stimulation approach: preliminary in vitro study

Jeeun Kang; Hanh N. D. Le; Haichong K. Zhang; Jin U. Kang; Maged M. Harraz; Emad M. Boctor

doi:10.1117/12.2253296

20 July 2017 Initial proof-of-concept of photoacoustic cell stimulation approach: preliminary in vitro study

Jeeun Kang, Hanh N. D. Le, Haichong K. Zhang, Jin U. Kang, Maged M. Harraz, Emad M. Boctor

Proceedings Volume 10052, Optogenetics and Optical Manipulation; 100520U (2017) https://doi.org/10.1117/12.2253296
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Localized, non-invasive cell stimulation has many applications. Here we show the initial proof-of-concept in vitro study of the photoacoustic cell stimulation approach, which is amenable to high-throughput screening applications. The proposed method is implemented as follows: 1) the localized excitation using the focused pulsed laser delivery on an absorptive material placed inside the well containing the cells, 2) cell stimulation by the photoacoustic pressure generated, and 3) fluorescence quantification of the membrane potential change over time. The preliminary proof-ofconcept in vitro study is conducted with primary neurons isolated from mouse cerebral cortex. The absorptive rubber media generates the photoacoustic pressure by the pulsed laser excitation. The experimental results show the feasibility of photoacoustic cell stimulation approach by indicating the significant membrane potential chance from the photoacoustically-stimulated primary neurons. Otherwise, the sham control without any photoacoustic stimulation shows minimal membrane potential change. We envisage that the proposed approach can allow broad strategies for noninvasive cell stimulation by using the photoacoustic contrasts situated at inside or outside of the body such as external absorptive materials or intravascularly-injected photoacoustic contrast particles.

Citation Download Citation

Jeeun Kang, Hanh N. D. Le, Haichong K. Zhang, Jin U. Kang, Maged M. Harraz, and Emad M. Boctor "Initial proof-of-concept of photoacoustic cell stimulation approach: preliminary in vitro study", Proc. SPIE 10052, Optogenetics and Optical Manipulation, 100520U (20 July 2017); https://doi.org/10.1117/12.2253296

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KEYWORDS

Photoacoustic spectroscopy

Neurons

In vitro testing

Pulsed laser operation

Brain

Luminescence

Optical manipulation

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