Advanced imaging approaches for characterizing nanoparticle delivery and dispersion in skin (Conference Presentation)

Tarl W. Prow; Miko Yamada; Nhung Dang; Conor L. Evans

doi:10.1117/12.2256789

19 April 2017 Advanced imaging approaches for characterizing nanoparticle delivery and dispersion in skin (Conference Presentation)

Tarl W. Prow, Miko Yamada, Nhung Dang, Conor L. Evans

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Proceedings Volume 10046, Visualizing and Quantifying Drug Distribution in Tissue; 1004607 (2017) https://doi.org/10.1117/12.2256789
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The purpose of this research was to develop advanced imaging approaches to characterise the combination of elongated silica microparticles (EMP) and nanoparticles to control topical delivery of drugs and peptides. The microparticles penetrate through the epidermis and stop at the dermal-epidermal junction (DEJ). In this study we incorporated a fluorescent lipophilic dye, DiI, as a hydrophobic drug surrogate into the nanoparticle for visualization with microscopy. In another nanoparticle-based approach we utilized a chemically functionalized melanin nanoparticle for peptide delivery. These nanoparticles were imaged by coherent anti-Stoke Raman scattering (CARS) microscopy to characterize the delivery of these nanoparticles into freshly excised human skin. We compared four different coating approaches to combine EMP and nanoparticles. These data showed that a freeze-dried formulation with cross-linked alginate resulted in 100% of the detectable nanoparticle retained on the EMP. When this dry form of EMP-nanoparticle was applied to excised, living human abdominal skin, the EMP penetrated to the DEJ followed by controlled release of the nanoparticles. This formulation resulted in a sustained release profile, whereas a freeze-dried formulation without crosslinking showed an immediate burst-type release profile. These data show that advanced imaging techniques can give unique, label free data that shows promise for clinical investigations.

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Tarl W. Prow, Miko Yamada, Nhung Dang, and Conor L. Evans "Advanced imaging approaches for characterizing nanoparticle delivery and dispersion in skin (Conference Presentation)", Proc. SPIE 10046, Visualizing and Quantifying Drug Distribution in Tissue, 1004607 (19 April 2017); https://doi.org/10.1117/12.2256789

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KEYWORDS

Nanoparticles

Skin

Microscopy

Visualization

CARS tomography

Coating

Silica

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