A frequency domain reconstruction of SIM image using four raw images

Amit Lal; XiaoShuai Huang; Peng Xi

doi:10.1117/12.2246242

31 October 2016 A frequency domain reconstruction of SIM image using four raw images

Amit Lal, XiaoShuai Huang, Peng Xi

Proceedings Volume 10024, Optics in Health Care and Biomedical Optics VII; 1002411 (2016) https://doi.org/10.1117/12.2246242
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

This work is a result of an exploration in response to the following observation: though reconstruction methodology of SIM image since its origin is carried out in frequency domain, recent works in this field, specially when it comes to SIM reconstruction using lesser than 9 raw images, have ventured into reconstruction methodologies that operate directly in the spatial domain. This work formulates and demonstrates a frequency domain reconstruction of SIM image using four raw images – one wide-field image and three SIM images. One of the chief feature of the presented reconstruction algorithm is that it employs the standard ‘tools and tricks’ used by the conventional 9-frame SIM reconstruction algorithm. Results indicate that the presented reconstruction algorithm provides high resolution reconstructions successfully as long as the noise level in the raw images is lower than 10%. For higher level of noise, the reconstruction result shows little resolution enhancement.

Citation Download Citation

Amit Lal, XiaoShuai Huang, and Peng Xi "A frequency domain reconstruction of SIM image using four raw images", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 1002411 (31 October 2016); https://doi.org/10.1117/12.2246242

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