Distributions In Phytoplankton Refractive Index And Size Within The North Sea

Steven G Ackleson; David B Robins; John A Stephens

doi:10.1117/12.945739

12 August 1988 Distributions In Phytoplankton Refractive Index And Size Within The North Sea

Steven G Ackleson, David B Robins, John A Stephens

Proceedings Volume 0925, Ocean Optics IX; (1988) https://doi.org/10.1117/12.945739
Event: 1988 Technical Symposium on Optics, Electro-Optics, and Sensors, 1988, Orlando, FL, United States

Abstract

A Coulter EPICS 741 Flow Cytometer was used at sea to measure distributions in phytoplankton refractive index and size within the North Sea. The optical data gathered by the flow cytometer, multi-band fluorescence and multi-angle light scatter, agreed well with coincident measurements of in vivo fluorescence. By gating on fluorescence, unique subpopulations of phytoplankton may be isolated optically from all other particles within a natural population. At several coastal stations, a unique subpopulation of phytoplankton was found which appeared to contain both chlorophyll and phycoerythrin. Using Mie scatter theory and flow cytometric measurements of light scatter in the near-forward and side directions, this population was found to be composed of cells of refractive index between 1.07 and 1.095 and spherical diameter between 7 and 9 pm (verified using Coulter volume measurements).

Citation Download Citation

Steven G Ackleson, David B Robins, and John A Stephens "Distributions In Phytoplankton Refractive Index And Size Within The North Sea", Proc. SPIE 0925, Ocean Optics IX, (12 August 1988); https://doi.org/10.1117/12.945739

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