In Vivo Measurements On Human Lens Using Quasielastic Light Scattering

Peter C. Magnante; Leo T. Chvlack; George B. Benedek

doi:10.1117/12.956356

5 August 1986 In Vivo Measurements On Human Lens Using Quasielastic Light Scattering

Peter C. Magnante, Leo T. Chvlack, George B. Benedek

Proceedings Volume 0605, Optical and Laser Technology in Medicine; (1986) https://doi.org/10.1117/12.956356
Event: O-E/LASE'86 Symposium, 1986, Los Angeles, CA, United States

Abstract

Quasi-elastic light scattering is a useful method to determine the size distribution of sub-micron particles in fluids. We have applied this technique to measure in vivo changes in the association of human lens protein constituents that occur with aging and cataractogenesis. The autocorrelation function of the scattered light has been analyzed by a two component exponential where each component is characterized by an intensity and decay rate. Each pair of parameters is associated with one of two major protein components in the lens. These parameters have been determined as a function of age and position within normal, clear lenses. In cataractous lenses the intensity and decay rate parameters show anomalous behavior, when compared to normal lenses, even in clear regions of the lens which are some distance from the cataract. Our results seem to be consistent with the model of progressive aggregation of the lens proteins as detected in vitro by other biochemical methods. Our studies suggest that quasi-elastic light scattering spectroscopy is a useful probe of the protein modifications that occur both in normal and cataractogenic human lenses.

Citation Download Citation

Peter C. Magnante, Leo T. Chvlack, and George B. Benedek "In Vivo Measurements On Human Lens Using Quasielastic Light Scattering", Proc. SPIE 0605, Optical and Laser Technology in Medicine, (5 August 1986); https://doi.org/10.1117/12.956356

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