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13 March 2024 Multiphoton imaging and control of melanopsin activation in living cellular systems by spectral-temporal modulation of a supercontinuum source
Carlos A. Renteria, Jiho Kahng, Brian Tibble, Rishyashring R. Iyer, Kayvan F. Tehrani, Yuan-Zhi Liu, Stephen A. Boppart
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Proceedings Volume PC12863, Quantum Effects and Measurement Techniques in Biology and Biophotonics; PC1286308 (2024) https://doi.org/10.1117/12.3001042
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Melanopsin, a tri-stable photopigment found in intrinsically-photosensitive retinal ganglion cells (ipRGCs), drives circadian rhythms and other non-image forming functions in the nervous system. Despite increased understanding of the biomolecular and spectroscopic properties of melanopsin, its multiphoton and ultrafast optical absorption properties remain underexplored. We demonstrate the effects of two-photon absorption of melanopsin using 900-1160 nm optical stimulation. Excitation in this bandwidth causes consistent increases in calcium levels in transfected HEK293T cells. Our results demonstrate the first reported nonlinear optical properties and corresponding functional responses of two-photon excitation of melanopsin in vitro, along with the effects of spectral-phase modulation on activation.
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Carlos A. Renteria, Jiho Kahng, Brian Tibble, Rishyashring R. Iyer, Kayvan F. Tehrani, Yuan-Zhi Liu, and Stephen A. Boppart "Multiphoton imaging and control of melanopsin activation in living cellular systems by spectral-temporal modulation of a supercontinuum source", Proc. SPIE PC12863, Quantum Effects and Measurement Techniques in Biology and Biophotonics, PC1286308 (13 March 2024); https://doi.org/10.1117/12.3001042
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KEYWORDS
Calcium
In vitro testing
Absorption
Retina
Phase shift keying
Pulse signals
Spectroscopy
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