Rapid dual-views projection microscopy with two-photon glutamate uncaging for neural imaging

Dongli Xu; Jun B. Ding; Leilei Peng

doi:10.1117/12.3003734

13 March 2024 Rapid dual-views projection microscopy with two-photon glutamate uncaging for neural imaging

Dongli Xu, Jun B. Ding, Leilei Peng

Proceedings Volume PC12828, Neural Imaging and Sensing 2024; PC1282803 (2024) https://doi.org/10.1117/12.3003734
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Here, we present a rapid dual-view projection imaging method with two-photon glutamate uncaging capability based on an upright Bessel beam light-sheet microscopy setup. Compared with light-sheet imaging, our projection imaging method can significantly improve the volumetric rate for neural imaging. In addition, an independent laser scanning module is designed for 2P uncaging, allowing simultaneous synaptic resolution of stimulation and 100 Hz volumetric imaging of neural activity in deep tissue. Imaging results from mouse brain slices under 2P glutamate uncaging will be presented.

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Dongli Xu, Jun B. Ding, and Leilei Peng "Rapid dual-views projection microscopy with two-photon glutamate uncaging for neural imaging", Proc. SPIE PC12828, Neural Imaging and Sensing 2024, PC1282803 (13 March 2024); https://doi.org/10.1117/12.3003734

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KEYWORDS

Microscopy

3D projection

Brain tissue

Stereoscopy

Bessel beams

Imaging systems

Microscopes

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