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Femtosecond laser direct write (fs-LDW) is a promising technique for fine 3D printing of biomaterials such as protein due to nonlinear multiphoton absorption processes facilitating microfabrication along a designated laser light path. Proteinaceous microstructures fabricated by fs-LDW are reported to retain their native protein function. Combined with submicron feature sizes, they might offer diverse biomedical or biochip applications. Here, we show the pulse energy dependence of free-form pure proteinaceous line segments fabricated by fs-LDW. To utilize fs-LDW for arbitrarily created complex shapes, it is important to minimize line segment mismatches and assure sufficient connectivity by carefully selecting the appropriate parameters.
Daniela Serien,Koji Sugioka, andAiko Narazaki
"Pulse energy dependence of free-form pure proteinaceous line fabrication by femtosecond laser direct write", Proc. SPIE PC12408, Laser Applications in Microelectronic and Optoelectronic Manufacturing (LAMOM) XXVIII, PC124080I (17 March 2023); https://doi.org/10.1117/12.2648815
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Daniela Serien, Koji Sugioka, Aiko Narazaki, "Pulse energy dependence of free-form pure proteinaceous line fabrication by femtosecond laser direct write," Proc. SPIE PC12408, Laser Applications in Microelectronic and Optoelectronic Manufacturing (LAMOM) XXVIII, PC124080I (17 March 2023); https://doi.org/10.1117/12.2648815