At Langevin Institute, ESPCI Paris, we have developed a label-free approach to detect and track viruses in aquatic solutions using an interferometric microscopy approach working in transmission. By improving the experimental setup design, we have increased the sensitivity to detect viruses as small as 20nm in diameter, AAV viruses, which are of paramount importance for gene therapy. Furthermore, based on the variation of the interferometric signal along the axial position, we introduce a new way to perform single-shot volumetric imaging which enables high-precision 3D tracking.
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