Aseptic cell sorting is important for cell culture and downstream analyses of sorted cells. However, keeping the sort aseptic is challenging as the sheath fluid in a cell sorter may easily be contaminated with germs such as bacteria, yeasts, viruses, or fungi. Thus, a regular chemical cleaning of the whole fluidics system is required. However, this procedure is time consuming, and its success can hardly be verified. Here, we present a method for sheath fluid decontaminated by irradiation with UV-C light using a flow-through principle. With this principle, a 5 log10 reduction of bacteria in the sheath fluid was achieved.
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