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Particle sizing of biofluids, tissue samples, and therapeutic reagents is highly relevant for biomedical and clinical applications. Traditional dynamic light scattering (DLS) instruments are limited in their capability to measure optically transparent samples and are not conducive for particle sizing in samples that exhibit rich optical scattering and absorption. Here we demonstrate a new approach that utilizes polarization-dependent attributes of laser speckle to measure particle sizes in biosamples that span a range of optical scattering and absorption properties. We further apply this technique for particle sizing in homogenous controls, as well as biofluids such as platelet rich plasma and whole blood.
Zeinab Hajjarian Kashany andSeemantini K. Nadkarni
"Polarization attributes of laser speckle for particle sizing in clinical and biomedical applications (Conference Presentation)", Proc. SPIE PC12382, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023, PC123820A (15 March 2023); https://doi.org/10.1117/12.2651034
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Zeinab Hajjarian Kashany, Seemantini K. Nadkarni, "Polarization attributes of laser speckle for particle sizing in clinical and biomedical applications (Conference Presentation)," Proc. SPIE PC12382, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023, PC123820A (15 March 2023); https://doi.org/10.1117/12.2651034